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-Simple, fast protocol -GelTrack Loading Dye pre-mixed -High yield, high sensitivity -Precise amplification - hot-start technology ensures specific and efficient primer extension -Superior reproducibility and precision The AccuStart II Mouse Genotyping Kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals The kit combines Extracta DNA Prep for PCR - Tissue with AccuStart II GelTrack PCR SuperMix in a single kit. Extracta reagents allow rapid extraction of PCR ready DNA that can be used directly in PCR reactions eliminating time consuming or expensive purification steps. AccuStart II GelTrack PCR SuperMix has been optimized for genotyping applications that commonly utilize 3 or more primers in multiplex PCR reactions that allow amplification and analysis of two or more targets in a single reaction such as normal and mutant alleles of a gene knockout or that determine the presence or absence of transgene constructs in transgenic animals. AccuStart II GelTrack PCR SuperMix contains all components for PCR (except primers and template) simplifying reaction assembly, improving assay reproducibility, reliability and reducing the risk of contamination. The PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine endpoint PCR amplification of DNA fragments up to 4 kb and subsequent analysis by agarose gel electrophoresis. The PCR SuperMix additionally includes electrophoresis-tracking dyes that migrate at approximately 4kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94oC), the antibodies denature irreversibly, releasing fully active, unmodified Taq
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